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Asian Journal of Pharmaceutical Analysis
Year : 2019, Volume : 9, Issue : 4
First page : ( 199) Last page : ( 204)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2019.00033.4

Estimation of Nateglinide by using MBTH as a Chromogenic Reagent

Babu N. Raghavendra1,*, Padmavathi Y.1, Babu R. Swethan2, Unnisa Amreen1, Bhavana M.1, Kumar P. Ravi1

1Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana

2Department of Pharmaceutical Chemistry, SRM University, Chennai, Tamil nadu

*Corresponding Author E-mail: nayakaraghavendrababu@gmail.com

Online published on 31 December, 2019.

Abstract

Nateglinide is used as an oral anti-diabetic drug. The aim of the present study is to develop spectrophotometric method for the estimation of nateglinide by using MBTH reagent. Visible spectrophotometric method has been developed for quantification of nateglinide. The absorbance of green colored chromogen was measured at 634nm against appropriate reagent blank and the graph of absorbance against concentration was plotted. Beer's law was obeyed in the concentration range of 50–300μg/mL. The LOD and LOQ were found to be 11.78 μg/mL and 35.7 μg/mL respectively with coefficient of correlation 0.999. The proposed method has been validated for linearity, accuracy and precision as per ICH guidelines and applied for quantitative analysis of nateglinide using chromogenic reagent.

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Keywords

Nateglinide, MBTH reagent, Visible spectrophotometry, Beer's law, ICH guidelines.

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