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Asian Journal of Pharmaceutical Analysis
Year : 2018, Volume : 8, Issue : 3
First page : ( 157) Last page : ( 163)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2018.00029.7

Development of Rapid and Sensitive Reverse Phase High Performance Liquid Chromatography Method for Estimation of Ketorolac Tromethamine in Proniosomal Gel

Farooqui Nadeem Ahmed1,*, Singh Ravindra Pal1, Kar Mousumi2, Chhajed Mahavir3, Dumbwani Jacky3

1Department of Pharmaceutics, Suresh Gyan Vihar University, Mahal, Jagat Pura, Jaipur (RJ) India

2Department of Pharmaceutics, College of Pharmacy, IPS Academy, Indore (MP) India

3Department of Pharmacology, Indore Institute of Pharmacy, Opp IIM, Indore, Rau-Pithampur Road, Indore (MP) India

*Corresponding Author E-mail: nadeemimran2000@gmail.com

Online published on 24 October, 2018.

Abstract

A reliable, rapid and sensitive isocratic reverse phase high-performance liquid chromatography (RP-HPLC) method has been developed and validated for analysis of Ketorolac Tromethamine (KT) for the estimation and comparison of ketorolac tromethamine in proniosomal gel and marketed gel formulation. An isocratic separation was achieved on Shimizu C18 column (250 cm×4.6 mm×5 μm) at ambient temperature by optimizing the experimental conditions. The compound was separated with the mixture of methanol and water in the ratio of 60: 40 V/V, pH 3.0 was adjusted with 0.1% o-phosphoric acid as the mobile phase at flow of 1.5 mL min−1. The eluents were monitored at 306 nm using photo diode array detection. The average retention time of five readings was found to be 6.936 min. The system suitability parameters like theoretical plate count, tailing factor and relative standard deviation of five standard injections were within the limit. The method was validated according to ICH guidelines. Calibrations were linear over the concentration range of 50–150 μg mL−1 as indicated by correlation coefficient (r) of 0.999. The robustness of the method was evaluated by deliberately altering the chromatographic conditions. The developed method can be applicable for routine quantitative analysis.

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Keywords

RP-HPLC, Chromatography, Ketorolac tromethamine, proniosomal gel.

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