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Asian Journal of Pharmaceutical Analysis
Year : 2018, Volume : 8, Issue : 2
First page : ( 73) Last page : ( 77)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2018.00014.5

A Validated RP-HPLC Method for Simultanious Determination of Metformin and Canagliflozin in Pharmaceutical Formulation

Dr. Swamy Gandla. Kumara1,*, Lalitha R.2, Mounika Ch., Soumya B.2, Kumar D. Sudheer2

1Associate Professor and H.O.D Department of Pharmaceutical Analysis, Care College of Pharmacy, Oglapur (Vill), Damera, (Mdl), Warangal (Dist)-506001. Telangana, India

2Care College of Pharmacy, Department of Pharmaceutical Analysis, Oglapur (Vill), Damera, (Mdl), Warangal-Rural, (Dist)-506001, Telangana, India

*Corresponding Author E-mail: kumaraswamy.gandla@gmail.com, drkumaraswamygandla@gmail.com

Online published on 7 July, 2018.

Abstract

The proposed study a new validated RP-HPLC method has been developed for estimation of metformin and canagliflozin in bulk and pharmaceutical dosage form. The present method was a sensitive, precise and RP-HPLC method for analysis of metformin and canagliflozin. To optimize the mobile phase various combinations of organic solvents were used on Primesil C18 250 column, Then the mobile phase containing a mixture of Methanol and phosphate buffer in the ratio of 70: 30% v/v was selected at a flow rate of 1.0ml/min for developing the method and the peak with a good shape and resolution were found resulting in short retention time baseline stability and minimum noise. The retention time metformin and canagliflozin found to be 2.413 and 3.47 min respectively, quantitative linearity was obeyed in the concentration range of 50–250 μg/mL for metformin and 5–25 μg/mL for canagliflozin. The limit of detection and limit of quantitation were found to be 2.1μg/ml and 0.0372μg/ml (metformin), 4.13μg/ml and 0.112μg/ml (canagliflozin) respectively, which indicates the sensitivity of the method. The high percentage recovery indicates that the proposed method is highly accurate no interfering peaks were found in the chromatogram indicating that excipients used in tablet formulation didn't interfere with the estimation of the drug by the proposed HPLC method.

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Keywords

RP-HPLC, Method development, metformin, canagliflozin; ICH Validation.

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