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Asian Journal of Pharmaceutical Analysis
Year : 2018, Volume : 8, Issue : 1
First page : ( 13) Last page : ( 19)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2018.00003.0

Reverse Phase High-Performance Liquid Chromatographic Estimation and In vitro Cytotoxicity of Boswellic Acids on A-375 Melanoma Cancer Cell lines

 Neeta, Dureja Harish*

Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak-124001, India

*Corresponding Author E-mail: harishdureja@gmail.com

Online published on 2 June, 2018.

Abstract

The present investigation was aimed at extraction, characterization and in vitro cytotoxic effects of boswellic acids (KBA and AKBA) against A-375 Melanoma Cancer Cell lines. Methanolic extract of Boswellia serrata was prepared and gradient Reverse Phase High-Performance Liquid Chromatographic (RP-HPLC) method was used for the identification and quantification of boswellic acids in the gum resin extract. Presence of boswellic acids in the extract was further confirmed by TLC, FTIR and DSC. In vitro cytotoxicity of the extract was evaluated by 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay against A-375 Melanoma Cancer cells Quantitative estimation of boswellic acids by RP-HPLC method was found to be around 40.6 mg/gram and 32.3 mg/gram for KBA and AKBA, respectively. MTT assay showed cytotoxic potential of boswellic acids against melanoma cell lines with IC50 of 254.9 μg/mL with significant cytotoxic effects.

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Keywords

Boswellic acids, TLC, RP-HPLC, FTIR, MTT assay.

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