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Asian Journal of Pharmaceutical Analysis
Year : 2017, Volume : 7, Issue : 2
First page : ( 124) Last page : ( 131)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2017.00020.5

UHPLC: Applications in Pharmaceutical Analysis

Khan Hamid1,*, Ali Javed2

1 Associate Professor, JK College of Pharmacy, Bilaspur, Chhattisgarh India-495001

2Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi, India-110062

*Corresponding Author E-mail: hamdardhamid@yahoo.in

Online published on 18 September, 2017.

Abstract

High Performance Liquid Chromatography (HPLC) is a major analytical technique for qualitative and quantitative drug analysis. More than 90% of drugs prescribed in official pharmacopoeias are being analyzed HPLC. But due to the higher regulatory requirements such as more number of samples, speed of analysis, and sensitivity of the method of analysis. The pharmaceutical industries adopting the more advanced chromatographic techniques. Ultra-high Performance Liquid Chromatography (UHPLC) offers an advancement of HPLC which is based on the principal of use of stationary phase consisting of particles less than 2μm. By using smaller particles, speed of analysis, peak capacity can be extended to new limits and the sample can be analyzed in a shorter period of time. The UHPLC technique is a new approach in the chromatographic separations and has been successfully employed for fast, high resolution separations with required sensitivity. This review provides the brief introduction and applications of UHPLC in the pharmaceutical analysis.

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Keywords

UHPLC, UPLC, Introduction, Applications, Pharmaceutical Analysis.

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