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Asian Journal of Pharmaceutical Analysis
Year : 2016, Volume : 6, Issue : 2
First page : ( 77) Last page : ( 82)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2016.00012.0

Simultaneous determination of Saxagliptin Hydrochloride and Glibenclamide in Synthetic Mixture using Spectrophotometric technique (First order Derivative Method)

Sisode Pranali S.1,*, Raj Hasumati A.1, Jain Vineet C.2

1Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, India

2Department of Pharmacognosy, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, India

*Corresponding Author E-mail: pranalisisode19@gmail.com, drharaj@yahoo.com

Online published on 21 July, 2016.

Abstract

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Saxagliptin Hydrochloride and Glibenclamide in synthetic mixture using first order derivative zero-crossing method. Saxagliptin Hydrochloride showed zero crossing point at 315.00nm while Glibenclamide showed zero crossing point at 229.40nm. The dA/dλ was measured at 229.40nm for Saxagliptin Hydrochloride and 315.00nm for Glibenclamide and calibration curves were plotted as dA/dλ versus concentration, respectively. The method was found to be linear (r2>0.9995) in the range of 5–25μg/ml for Saxagliptin Hydrochloride at 229.40nm. The linear correlation was obtained (r2>0.9994) in the range of 5–25 μg/ml for Glibenclamide at 315.00nm. The limit of determination was 0.243μg/ml and 0.317μg/ml for Saxagliptin Hydrochloride and Glibenclamide, respectively. The limit of quantification was 0.738μg/ml and 0.960μg/ml for Saxagliptin Hydrochloride and Glibenclamide respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Saxagliptin Hydrochloride and Glibenclamide in binary mixture.

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Keywords

Saxagliptin Hydrochloride, Glibenclamide, First Derivative Method, Spectroscopic method.

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