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Asian Journal of Pharmaceutical Analysis
Year : 2020, Volume : 10, Issue : 2
First page : ( 109) Last page : ( 111)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2020.00019.8

Development and Validation of RP-HPLC Method for the Estimation of Bilastine from bulk and Formulation

Pardeshi Payal J.*, Gaware V. M., Dhamak K. B.

Department of Quality Assurance, Pres's College of Pharmacy (For Women), Chincholi, Tal: Sinnar, Dist: Nashik (MS) India

*Corresponding Author E-mail: payalp0220@gmail.com

Online published on 3 June, 2020.


A Simple, linear, precise, accurate, robust and selective RP-HPLC method has been developed for the estimation of Bilastine from bulk and tablet formulation. The methanol: orthophosporic acid buffer in proportion of (70: 30) was used as mobile phase. The HPLC system consisting of LC20AD Prominence Liquid Chromatography SPD 20-A Shimadzu Japan. The UV-VIS detector and C18 column with dimension on 250×4.6 mm was used at wavelength 280 nm. Finally Bilastine was quantified from bulk and its tablet formulation. The proposed method was found to be linear, precise and accurate as per the ICH guidelines.



Bilastine, RP-HPLC, Validation, Method development.


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