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Applied Biological Research
Year : 2011, Volume : 13, Issue : 2
First page : ( 117) Last page : ( 120)
Print ISSN : 0972-0979. Online ISSN : 0974-4517.

Status of apple mosaic virus in Kashmir Valley

Padder B.A.*, Shah M.D., Ahmad Mushtaq, Hamid Aflaq, Sofi T.A., Ahanger F.A., Saleem Sahar

Division of Plant Pathology, S.K. University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir 191 121, India

*e-mail: bapadder@rediffmail.com

Online published on 10 November, 2011.

Received:  14  April,  2011; Accepted:  5  September,  2011.

Abstract

Leaves of apple cv. ‘Red Delicious’ and ‘Golden Delicious’ were randomly collected from commercial apple growing areas of four districts of Jammu and Kashmir State viz., Shopian, Pulwama, Bandipora and Kupwara for the detection of Apple mosaic virus (ApMV) by RT-PCR technique using coat protein gene-specific primers. ApMV was present in all the locations. RT-PCR amplified approximately 800 bp amplicons from 30 out of 276 leaf samples thereby confirmed the infection of ApMV in Kashmir. District Bandipora recorded highest disease incidence of 13.3% followed by Shopian (11.3%), Kupwara (10.2%) and Pulwama (8.6%). Nineteen of the 161 cv. ‘Red Delicious’ samples and 11 of the 115 cv. ‘Golden Delicious’ samples were found RT-PCR positive.

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Keywords

Apple mosaic virus, ApMV incidence, Kashmir, RT-PCR detection.

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